FLIM (Fluorescence Lifetime Imaging Microscopy) is an advanced fluorescence imaging technique that allows for the measurement of the fluorescence lifetime of a fluorophore at each pixel of an image. In FLIM, a pulsed laser is used to excite the sample and a specialized detector is used to measure the time delay between the excitation and emission of the fluorescence signal. This time delay is proportional to the fluorescence lifetime of the fluorophore, which can provide important information about the local environment and interactions of the molecule. FLIM has become an important tool in a variety of fields, including biophysics, cell biology, and neuroscience.